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. Author manuscript; available in PMC: 2018 Sep 21.
Published in final edited form as: Mol Cell. 2017 Aug 31;67(6):1013–1025.e9. doi: 10.1016/j.molcel.2017.07.028

Figure 1.

Figure 1

Dynamics of key regulators during the DNA damage response. Levels of (A) p53 protein (n = 5), (C) Mdm2 protein (n = 3), and (E) MYC protein (n = 5) were measured by western blotting in MCF-7 cells treated with NCS. Levels of (B) MDM2 mRNA (n = 4) and (D) MYC mRNA (n = 4) were measured by qPCR in MCF-7 p53-Venus cells treated with NCS, as described previously (Porter et al., 2016). Levels of (F) p53 protein (n = 3) and (H) MYC protein (n = 3) were measured by western blotting in p53-knockdown cells (MCF-7 sh-p53) treated with NCS. Levels of (G) MYC mRNA (n = 4) were measured by qPCR in MCF-7 sh-p53 cells treated with NCS, as described previously (Porter et al., 2016). Measurements in (F–H) are normalized to those in untreated control cells (MCF-7 pSuper) (Brummelkamp et al., 2002). Gray backgrounds denote early response prior to p53-driven response. Data are represented as mean ± SEM.