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A cell system to control MYC expression. (A) MCF-7 variants with doxycycline-inducible constructs, including schematics of desired MYC expression in cell lines. (B) MYC dynamics in MYC-GFP cells during the DSB response. Levels of MYC protein were measured by western blotting in MYC-GFP cells treated with NCS; bands for endogenous MYC and MYC-GFP were quantified separately. Gray backgrounds denote early response prior to p53-driven response. Data are represented as mean ± SEM (n = 3).
