Protein abundance of (a) Rhesus blood group-associated glycoprotein (Rhag), (b) Rhesus family B glycoprotein (Rhbg) and (c) Rhesus family C glycoprotein (Rhcg) in the gills of Protopterus annectens kept in fresh water on day 0 (FW; control), or after 6 days (d; the induction phase) or 6 months (mon; the maintenance phase) of aestivation, or after 1 or 3 d of arousal (Ar; the arousal phase) from 6 mon of aestivation. (i) Examples of immunoblot of Rhag, Rhbg or Rhcg. (ii) The protein abundance of Rhag, Rhbg or Rhcg expressed as arbitrary densitometric units per 100 μg, 100 μg or 50 μg protein, respectively. Results represent mean ± S.E.M. (N = 4). Means not sharing the same letter are significantly different (P<0.05).