Figure 1.

Inhibitory effect of BIO on GSK-3β in the mouse brain. (A-C) Western blot analysis of 6-bromoindirubin-3′-oxime (BIO) target glycogen synthase kinase-3β (GSK-3β) was performed using tissue lysate from the perihemorrhagic regions following mouse intracerebral hemorrhage (ICH). (A) Representative immunoblots of all groups are shown. (B) Quantification of immunoblot intensities of total GSK-3β. (C) Quantification of immunoblot intensities of phospho-GSK-3β (Y216 residue). (D) Comparison of relative volumes of hematoma (mm³) in ICH animals treated with BIO. Mean+standard error of the mean (SEM). ^∗p < 0.05 compared to ICH-only group. n = 6–8 per group.