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. 2017 May 1;26(5):773–787. doi: 10.3727/096368916X692654

Figure 2.

Figure 2.

The attachment ability of human embryonic stem cells (hESCs) cryopreserved with SCK or DAP and cultured on a feeder cell layer. Freeze-thawed hESCs were cultured on a layer of SNL feeder cells, and their attachment to the bottom of a culture dish was observed under an inverted microscope. hESC colonies were photographed at 40x magnification. The number of colonies was counted by marking them with dots. hESC colonies after cryopreservation with SCK (a-d) or DAP (e-h) and culturing for 1 day (Day1). hESC colonies after cryopreservation with SCK (i-l) or DAP (m-p) and culturing for 2 days (Day2). (q) Comparison of the number of hESC colonies after cryopreservation with SCK or DAP and culturing for 1 or 2 days. Data are presented as means ± SD. Statistical evaluations were done by the Tukey-Kramer test. *p < 0.05, statistically significant difference. hESC colonies after cryopreservation with SCK (r-u) or DAP (v-y) and culturing for 5 days (Day5) (r-y). Both 5-day cultures had many colonies, and the numbers were not determined. Scale bars: 100 um. CPA, cryoprotective agent.