Figure 3.

The ability of hESCs cryopreserved with SCK or a solution of 2 M dimethyl sulfoxide (DMSO), 1 M acetamide, and 3 M DAP to attach to the bottom of Matrigel-coated culture dishes. Freeze-thawed hESCs were cultured on a layer of feeder cells for several days, and then disaggregated and cultured on Matrigel-coated dishes for 1 day. Cells were then observed for their adherence under an inverted microscope. hESC colonies were photographed at 40x magnification. The number of colonies was counted by marking them with dots. hESC colonies had a star-shaped morphology. hESCs after 1 day (d1) of cryopreservation with (SCK) (a-c) or DAP (d-f). (g) Comparison of the number of hESC colonies following cryopreservation with SCK or DAP. Data are presented as means ± SD. Statistical evaluations were done by Student's t-test. *p < 0.05, statistically significant difference. Scale bars: 100 um. CPA, cryoprotective agent.