Table 1.
Comparison of the Threshold Cycle (Ct) Between GAPDH and SOX2 for RT-PCR Analyses of hESCs Cryopreserved With SCK or DAP
| (1) The 2-Ct value ratio of GAPDH between SCK and DAP | |||
|---|---|---|---|
| Ct (SCK) | 2-Ct | Ct (DAP) | 2-Ct |
| 15.33 | 2.43E-05 | 16.75 | 9.04E-06 |
| 15.23 | 2.60E-05 | 16.55 | 1.05E-05 |
| 15.22 | 2.63E-05 | 16.56 | 1.03E-05 |
| Mean ± SD | (2.6E-05) ± (1.1E-06) | (9.9E-06) ± (7.8E-07) | |
| 2-Ct SCK/2-Ct DAP = 2.6 | |||
The comparative Ct method requires a stable reference gene for evaluating mRNA expression. The 2-Ct SCK/2-Ct DAP ratios of the GAPDH and SOX2 genes were obtained from the RT-PCR data. The ratio with SOX2 was more stable than that with GAPDH and was used as the control gene.