Figure 4.

The expression level of miR-210 in hADMSCs is directly regulated by cellular ROS production and ZD treatment. (A, B) The expression of miR-210 was dose-dependently induced by ROS generator antimycin or rotenone after different incubation durations. (C) miR-210 expression was measured in hADMSCs when treated with antimycin, rotenone, LPS/H₂O₂ with or without NAC, a kind of ROS scavenger. (D) ZD cotreatment successfully reduced the elevated miR-210 expression by antimycin or rotenone. **p < 0.01, ***p < 0.001 mean significant changes between control and treatment groups, respectively; ##p < 0.01, ###p < 0.001 mean significant changes between added NAC and non-added NAC group or between nontreated and treated with ZD group, respectively. Ctrl, control; ZD, zeaxanthin dipalmitate; hADMSCs, human adipose-derived mesenchymal stem cells; LPS, lipopolysaccharide; NAC, N-acetyl-L-cysteine; miR-210, microRNA-210.