Figure 6.

Maintenance of normal miR-210 expression is critical for cell homeostasis of hADMSCs. (A) We constructed replication-deficient lentivirus-encoding human miR-210 precursor or inhibitor sponge to overexpress (OE) or knock down (KD) the endogenous expression of miR-210 in hADMSCs, respectively. (B, C) Cell viability and apoptotic ratio were measured after OE or KD of miR-210 in stem cell pretreated with LPS/H₂O₂ or not, in the absence or presence of ZD. (D) Treated OE or KD of miR-210 in stem cell with LPS/H₂O₂ with or without ZD altered the level of miR-210. Dark gray column: LPS/H₂O₂ coincubation, dark columns: overexpressed miR-210 with or without LPS/H₂O₂ treatment, gray columns: LPS/H₂O₂ + overexpressed miR-210 + ZD cotreatment, light gray columns: knocked down miR-210 with or without LPS/H₂O₂ treatment. *p < 0.05, **p < 0.001 mean significant changes between control and indicated treatment groups, respectively; #p < 0.05, ##p < 0.01, ###p < 0.001 mean significant changes between LPS/H₂O₂ treatment group and OE/KD miR-210 group, respectively; @p < 0.05, @@p < 0.01, @@@p < 0.001 mean significant change between non-treated and treated with ZD group, respectively. Ctrl, control; miR, microRNA; LPS, lipopolysaccharide; ZD, zeaxanthin dipalmitate; hADMSCs, human adipose-derived mesenchymal stem cells.