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. 2017 Jun 30;26(7):1262–1275. doi: 10.1177/0963689717715168

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Morphology and identification of neural stem cells (NSCs). (a) The morphology of cells at 12 h of primary culture. (b) The neurospheres composed of hundreds of cells at 3 d after culture under microscope in bright field. (c, d, and e) Three days after culture, respectively. Nestin (marker of NSCs), NeuN (marker of neurons), and glial fibrillary acidic protein (GFAP; marker of astrocytes) immunopositive cells were observed under the light microscope, respectively. Scale bar = 25 μm in a, b, d, e. Scale bar = 50 μm in c.