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. 2017 Jun 30;26(7):1276–1285. doi: 10.1177/0963689717716510

Table 3.

Primer Names, Sequences, and PCR Reaction Conditions.

Gene Primers Temperature (°C)
Trim32 (rats) Forward: 5′ CGGAGCATGGAAGTCACAG 3′ 60
Reverse: 5′ ACCACAGCCAGGAAACCC 3′
Tnr (rats) Forward: 5′ CCT GGT CGG GAA CAA AGT 3′ 60
Reverse: 5′ TTC ATC TGC CGC ACA ATT 3′
COX5A (rats) Forward: 5′ AGT TGG GAG GCT ATG TTG C 3′ 60
Reverse: 5′ TGC GGT TGG AAA TCT GTC 3′
Trim32 (mouse) Forward: 5′ GCATCCAGGAAGAGCTAG 3′ 60
Reverse: 5′ CTCTACCACTTGACTGTTG 3′
TAp73 (mouse) Forward: 5′ GCACCTACTTTGACCTCCCC 3′ 60
Reverse: 5′ GCACTGCTGAGCAAATTGAAC 3′
GAPDH (rats) Forward: 5′ GTC CTT GAT CAC CCG ATT C 3′ 60
Reverse: 5′ TCC TGT GTG CTT TCC ATT C 3′
β-Actin (rats) Forward: 5′ GAAGATCAAGATCATTGCTCCT 3′ 52
Reverse: 5′ TACTCCTGCTTGCTGATCCA 3′
GAPDH (mouse) Forward: 5′ TGTGTCCGTCGTGGATCTGA 3′ 60
Reverse: 5′ CCTGCTTCACCACCTTCTTGA 3′
β-Actin (mouse) Forward: 5′ TCACTATTGGCAACGAGCGGTTC 3′ 52
Reverse: 5′ GCACTGTGTTGGCATAGAGGTCTT 3′

Abbreviation: GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.