Table 3.
Gene | Primers | Temperature (°C) |
---|---|---|
Trim32 (rats) | Forward: 5′ CGGAGCATGGAAGTCACAG 3′ | 60 |
Reverse: 5′ ACCACAGCCAGGAAACCC 3′ | ||
Tnr (rats) | Forward: 5′ CCT GGT CGG GAA CAA AGT 3′ | 60 |
Reverse: 5′ TTC ATC TGC CGC ACA ATT 3′ | ||
COX5A (rats) | Forward: 5′ AGT TGG GAG GCT ATG TTG C 3′ | 60 |
Reverse: 5′ TGC GGT TGG AAA TCT GTC 3′ | ||
Trim32 (mouse) | Forward: 5′ GCATCCAGGAAGAGCTAG 3′ | 60 |
Reverse: 5′ CTCTACCACTTGACTGTTG 3′ | ||
TAp73 (mouse) | Forward: 5′ GCACCTACTTTGACCTCCCC 3′ | 60 |
Reverse: 5′ GCACTGCTGAGCAAATTGAAC 3′ | ||
GAPDH (rats) | Forward: 5′ GTC CTT GAT CAC CCG ATT C 3′ | 60 |
Reverse: 5′ TCC TGT GTG CTT TCC ATT C 3′ | ||
β-Actin (rats) | Forward: 5′ GAAGATCAAGATCATTGCTCCT 3′ | 52 |
Reverse: 5′ TACTCCTGCTTGCTGATCCA 3′ | ||
GAPDH (mouse) | Forward: 5′ TGTGTCCGTCGTGGATCTGA 3′ | 60 |
Reverse: 5′ CCTGCTTCACCACCTTCTTGA 3′ | ||
β-Actin (mouse) | Forward: 5′ TCACTATTGGCAACGAGCGGTTC 3′ | 52 |
Reverse: 5′ GCACTGTGTTGGCATAGAGGTCTT 3′ |
Abbreviation: GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.