Figure 1.

Effect of different cell-detaching procedures on cell recovery and cell viability. One hundred thousand cells were seeded on 15-cm dishes and maintained for 2 weeks. (A) Number of the cells recovered by each cell-detaching reagent and incubation time indicated. (B) Number of cells recovered by each cell-detaching reagent at 5 min. (C) Detached cells were stained with 7-aminoactinomycin D (7AAD), and living cell populations were calculated by flow cytometry. (D) Survival rate of the detached mesenchymal stem cells (MSCs) by each reagent at 30 min. Data are represented as the average and standard deviation of six donors. ^*p < 0.05. N.E., not examined; n.s., not significant.