Figure 3.

Surface antigen expression of detached MSCs. One hundred thousand cells were seeded on 15-cm dishes and maintained for 2 weeks. Cell suspensions were prepared using detaching reagents indicated. Detached cells were stained with antibodies as listed in the figure. Each antigen-positive population was calculated by a flow cytometer. (A) Positive cell population of each surface antigen after trypsin or TrypLE treatment for 5 min. CD140a⁺ cell population was quickly and significantly reduced by trypsin treatment. (B) Positive cell population of each surface antigen after four different cell-detaching reagent treatments for 30 min. CD140a⁺ cell population was significantly reduced by trypsin, collagenase, and C5789 treatment. Data are presented as the average and standard deviation of six donors. ^*p < 0.05.