Skip to main content
. 2017 Feb 1;26(2):173–189. doi: 10.3727/096368916X692212

Figure 3.

Figure 3

Human ADSCs induce M2 macrophages, inhibit neutrophil transmigration, and suppress lymphocyte activation in vitro. (A) Adipose-derived stem cells (ADSCs) increased the percentage of M2 macrophages (CD14+CD206+/total CD14+) in human peripheral blood-derived mononuclear cell (PBMNC) 3-day Transwell cultures with or without tumor necrosis factor-α (TNF-α) in a concentration-dependent manner. (B) Representative flow cytometry plot. (C) ADSCs cocultured with human PBMNCs had an increased percentage of regulatory T cells (Tregs). (D) Representative CD4-gated flow cytometry plot. (E) Neutrophil transmigration assay cartoon. (F) ADSCs inhibited TNF-α-induced neutrophil migration across an endothelial cell (EC) monolayer. (G) Carboxyfluorescein succinimidyl ester (CFSE)-labeled neutrophils. (H) ADSCs and ADSC-conditioned media (ADSC-CM) dose-dependently attenuated anti-CD3- and anti-CD28-activated human PBMNC proliferation compared to nonactivated PB-MNCs. ap < 0.05 versus control; bp < 0.05 versus vehicle. n = 3–4/group. (A, F, H) ANOVA with post hoc Newman–Keuls test; (C) Student's t-test.