Figure 2.

Polymerase chain reaction (PCR) showing an efficient removal of bacterial contamination by the processing procedure. Real-time PCR against 16s rRNA regions conservative for the entire bacterial kingdom [targeted by the BactQuant (A) and Jordan (B) primers], and regions specific for Lactobacillus (C) and human DNA (D). Abscissa: PCR cycle. Ordinate: relative fluorescence units (RFU). ENTRY: transportation medium used for harvesting the fetal material at the operation site. Liver, brain, soft tissues: samples prepared from the entry material. Mock: a water sample passed through the DNA isolation procedure to serve as a negative control. Note the much earlier rise of 16s rRNA PCR kinetics for “entry” in comparison to the prepared samples witnessing a dramatic reduction of bacterial DNA content after processing. The experiment has been repeated more than three times.