Skip to main content
. 2017 Feb 1;26(2):339–350. doi: 10.3727/096368916X692735

Figure 4.

Figure 4

Detection of bacterial DNA in processed fetal preparations by PCR. PCR against 16s rRNA regions conservative for the entire bacterial kingdom [targeted by the BactQuant (A) and Jordan (B) primers]. Abscissa: PCR cycle. Ordinate: relative fluorescence units (RFU). Gray curves: dilutions of Escherichia coli (E. coli) DNA were used as standards. 1, 2, 3, 4: log genome equivalents per reaction. Dark curves: PCR from clinical samples labeled as “contaminated” and “clean” following the criteria described in the text. Mock: a water sample passed through the DNA isolation procedure to serve as a negative control. Insets: gel electrophoresis with BactQuant (447 bp) and Jordan PCR (371 bp) products from mock and clinical samples. Molecular weight is given in kilobases (kbp).