Table 1. Primary and secondary antibodies used for immunohistochemistry.
| Primary | Host | Clone # | Company | Catalog # | Dilution (fold) |
| α-amylase | Rabbit | Sigma Aldrich (St. Louis, MO) |
A8273 | 200 | |
| CD40 | Armenian hamster (IgM) | HM40-3 | eBioscience (San Diego, CA) |
14–0402 | 2,000 |
| CD206 | Rat | MR5D3 | AbD Serotec (Raleigh, NC) |
MCA2235GA | 500 |
| F4/80 | Rat | CI:A3-1 | BMA Biomedicals (Augst, Switzerland) |
T-2008 | 250 |
| Glucagon | Rabbit | Sigma Aldrich | SAB4501137 | 200 | |
| Iba-1 | Rabbit | Wako (Osaka, Japan) |
019–19741 | 500 | |
| Insulin | Guinea pig | Dako (Glostrup, Denmark) |
N1542 | 100 | |
| MHC class II | Rat | M5/114.15.2 | eBioscience | 14–5321 | 200 |
| Secondary | Host | Conjugation | Company | Catalog # | Dilution (fold) |
| Armenian hamster IgM | Mouse | Biotin | BD Biosciences (Franklin Lakes, NJ) |
554035 | 200 |
| FITC | 554033 | 200 | |||
| Guinea pig IgG |
Goat | Biotin | Vector Laboratories (Burlingame, CA) |
BA-7000 | 200 |
| Alexa488 | Thermo Fisher Scientific (Waltham, MA) | A11073 | 500 | ||
| Alexa546 | A11074 | ||||
| Alexa647 | A21450 | ||||
| Rabbit IgG | Goat | Biotin | Vector Laboratories | BA-1000 | 200 |
| Alexa488 | Thermo Fisher Scientific | A11034 | 500 | ||
| Alexa546 | A11035 | ||||
| Alexa647 | A21245 | ||||
| Rat IgG | Goat | Texas red | Jackson Laboratories (West Grove, PA) |
112-075-167 | 200 |
| FITC | 112-095-167 |
CD40, cluster of differentiation 40; CD206, cluster of differentiation 206; FITC, fluorescein isothiocyanate; Iba1, ionized calcium-binding adapter molecule 1; IgG, immunoglobulin G; IgM, immunoglobulin M; MHC, major histocompatibility complex