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. 2017 Oct 26;12(10):e0186303. doi: 10.1371/journal.pone.0186303

Fig 1. Effect of weight reduction on adipose tissue inflammation in obese mice.

Fig 1

Mice were fed either a high-fat diet (HFD) or a control diet (CD). The HFD was introduced at 4 weeks of age. Half of the HFD mice were switched to control diet at 30 weeks of age (HFD to CD). The other half of the HFD mice were continuously kept on the HFD. These mice were evaluated at 38 weeks of age. a, b Body and eVAT weights were analyzed (n = 6 mice per group). c, d Effect of HFD or weight reduction on oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) (n = 6 mice per group). *: CD vs HFD → CD, #: CD vs HFD. e Serum insulin levels of mice fed CD, HFD, and HFD to CD (n = 3–9 mice per group). f Adipose tissue of indicated mice were analyzed for the expression of Spp1, Tnf, Il1b, and Col1a1 by real-time PCR analysis (n = 6 mice per group). *P < 0.05, **P < 0.01, and ***P < 0.001, ##P < 0.01, and ###P < 0.001; n.s., not significant. Data are represented as mean ± SEM.