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. Author manuscript; available in PMC: 2017 Oct 26.
Published in final edited form as: Exp Cell Res. 2013 Jan 24;319(7):1001–1012. doi: 10.1016/j.yexcr.2013.01.012

Fig. 4.

Fig. 4

N-acetylcysteine prevents HG-induced ROS generation and DNA damage in retinal pericytes. (a) ROS generation. N-acetylcysteine (NAC, 5 mM) was added during the last 2 days of treatment while HG was present for 5 days. NAC significantly reduces HG-induced ROS generation in TR-rPCT1 (p<0.001; HG vs. HG+NAC). (b) IHC of DNA break and chromatin condensation (DAPI staining). HG induces TXNIP and chromatin breakage (middle panel, arrows). TXNIP up regulation and chromatin breakage by HG are reduced by NAC (right panel), which is more of less comparable to LG (left panel). A representative of n=3 is shown. (c) NAC also reduces COMET tailing by DNA break in HG treated pericytes suggesting a role for ROS in chromatin instability and breakage. A representative of n=3 is shown.