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. Author manuscript; available in PMC: 2018 Sep 21.
Published in final edited form as: Cell. 2017 Sep 7;171(1):217–228.e13. doi: 10.1016/j.cell.2017.08.006

Figure 1. Type 2 cytokines activate mouse and human sensory neurons.

Figure 1

(A) Representative gel of RT-PCR product of whole mouse dorsal root ganglia (DRG), n = 4 wild type (WT) mice.

(B) Representative gel of RT-PCR product of whole human DRG, N = 3 donors.

(C) Comparison of Il4ra, Il13ra1, and Il31ra expression by RT-qPCR in whole mouse DRG and trigeminal ganglia (TG), n = 4 WT mice.

(D–F) Representative calcium imaging trace of mouse DRG neurons in response to (D) recombinant murine (rm)IL-4 (300 nM), (E) rmIL-13 (300 nM), and (F) rmIL-31 (300 nM) and potassium chloride (KCl, 100 mM).

(G) rmIL-4-, rmIL-13-, rmIL-31-, and histamine-responsive DRG neurons as a percentage of total KCl-responsive neurons, n > 500 neurons from 2 WT mice.

(H) Representative calcium imaging trace of human DRG neurons in response to recombinant human IL-4 (300 nM), capsaicin (500 nM), and KCl (50 mM), n > 40 neurons from a male donor. Data are represented as mean ± SEM. Black bars in calcium imaging traces indicate timing of challenges. See also Figure S1.