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. Author manuscript; available in PMC: 2018 Sep 21.
Published in final edited form as: Cell. 2017 Sep 7;171(1):217–228.e13. doi: 10.1016/j.cell.2017.08.006

Figure 3. Neuronal IL-4Rα expression is necessary for chronic itch.

Figure 3

(A) Quantification of Il4ra, Il2rg, and Il13ra1 expression by RT-qPCR in whole sensory trigeminal ganglia (TG) of IL-4RαΔneuron mice, n ≥ 4 mice per group.

(B–E) Representative responses of DRG neurons from an IL-4RαΔneuron mouse to (B) rmIL-4 (1 μg/mL), (C) rmIL-13 (4 μg/mL), (D) rmIL-31 (3 μM), and (E) histamine (50 μM) as a percentage of total KCl-responsive neurons compared to neurons from a littermate control mouse, n > 300 neurons per group.

(F) Experimental schematic indicating daily topical treatment with MC903 to the ears of IL-4RαΔneuron and littermate control mice.

(G) Scratching behavior of IL-4RαΔneuron mice compared to littermate control mice over the course of MC903 treatment, n ≥ 7 mice per group.

(H) Ear thickness measurements, (I) representative H&E histopathology, and (J) histology score of MC903-treated IL-4RαΔneuron mice compared to littermate control mice, n ≥ 4 mice per group. Scale bars indicate 100 μm. Data are represented as mean ± SEM. See also Figures S3–S5.