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. Author manuscript; available in PMC: 2018 Sep 21.
Published in final edited form as: Cell. 2017 Sep 7;171(1):217–228.e13. doi: 10.1016/j.cell.2017.08.006

Figure 4. Disruption of neuronal JAK1 reduces chronic itch.

Figure 4

(A) Representative responses to rmIL-4 (1 μg/mL) of DRG neurons from a JAK1Δneuron mouse compared to a littermate control mouse as a percentage of total KCl-responsive neurons, n ≥ 200 neurons per group.

(B) Experimental schematic indicating MC903 treatment of JAK1Δneuron and littermate control mice.

(C) Scratching behavior, (D) ear thickness measurement, (E) representative H&E histopathology, and (F) histology score of JAK1Δneuron mice compared to littermate control mice on Day 7, n ≥ 8 mice per group.

(G) Representative responses to rmIL-4 (1 μg/mL) of DRG neurons from a WT mouse after incubation with ruxolitinib (Rux, 1 μg/mL) compared to vehicle control as a percentage of total KCl-responsive neurons, n ≥ 200 neurons per group.

(H) Experimental schematic indicating twice daily intraperitoneal (i.p.) injection of vehicle control or Rux (100 μg) during MC903 treatment to the ears of WT mice.

(I) Scratching behavior, (J) ear thickness measurement, (K) representative H&E histopathology, and (L) histology score of vehicle control and Rux-treated mice on Day 7, n ≥ 10 mice per group.

Scale bars indicate 100 μm. Data are represented as mean ± SEM. See also Figure S6.