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. 2017 Oct 26;12(10):e0187002. doi: 10.1371/journal.pone.0187002

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© 2017 Soto et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) SDS-PAGE of rTgPI-1 stained with Coomassie Blue. (B) Western blot of rTgPI-1 revealed with anti-His-Tag (lane 1) antibody or mouse anti-TgPI-1 serum (lane 2). (C) Analysis of inhibitory effect of rTgPI-1 on trypsin by gelatin substrate-SDS PAGE. All lanes contain 50 ng of trypsin. Lane 1, 1 μg of rTgPI-1; lane 2, control without rTgPI-1. Gelatinolytic activity was visualized by staining with Coomassie Brilliant Blue. The image was digitally inverted.