Table 1. Gene-specific primers for mRNA amplification of key lipid metabolism genes.
| Gene ID | Accession ID | Gene name | primer | Product length |
|---|---|---|---|---|
| 396526 | NM_205518 | β-actin | Forward: CTGTGCCCATCTATGAAGGCTA | 139 |
| Reverse: ATTTCTCTCTCGGCTGTGGTG | ||||
| 396061 | NM_205155 | FASN | Forward: GCAGCTTCGGTGCCTGTGGTT | 119 |
| Reverse: GCTGCTTGGCCCACACCTCC | ||||
| 396504 | NM_205505 | ACACA | Forward: AACGAGTCGGGCTACTACCT | 119 |
| Reverse: ATCAGCATCCCGTGAAGTGG | ||||
| 395706 | NM_204890 | SCD-1 | Forward: CCAGCGGAGATACTACAAGCC | 184 |
| Reverse: CCGATTGCCAAACATGTGAGC | ||||
| 373915 | NM_204126 | SREBF-1c | Forward: TCACCGCTTCTTCGTGGAC | 220 |
| Reverse: CTGAAGGTACTCCAACGCATC | ||||
| 423118 | NM_001012898 | CPT1A | Forward: TCGTCTTGCCATGACTGGTG | 143 |
| Reverse: GCTGTGGTGTCTGACTCGTT | ||||
| 417366 | NM_001006205 | ACOX1 | Forward: ATGTCACGTTCACCCCATCC | 133 |
| Reverse: AGGTAGGAGACCATGCCAGT | ||||
| 374120 | NM_001001464 | PPARα | Forward: TGTGGAGATCGTCCTGGTCT | 103 |
| Reverse: CGTCAGGATGGTTGGTTTGC |
Notes: β-actin, beta-actin; FASN, fatty acid synthase; ACACA, acetyl-CoA carboxylase; SCD1, stearoyl-coenzyme A desaturase 1; SREBF-1c, sterol regulatory element-binding protein-1c; CPT1A, carnitine palmitoyl transferase 1; ACOX1, acyl-CoA oxidase 1; PPARα, peroxisome proliferator-activated receptor-alpha.