Table 1. Peptides and transitions for quantification of ABCA1 and HDACs by mass spectrometry.
| Protein | Peptide | Precursor (m/z) |
Product ions (m/z) | ||||
|---|---|---|---|---|---|---|---|
| ABCA1 | ILYTPDTPATR | L | 624.3 | 545.3 (y5) | 757.4 (y7) | 858.4 (y8) | |
| H | 631.3 | 553.3 (y5) | 767.4 (y7) | 869.4 (y8) | |||
| HDAC1,2 | YGEYFPGTGDLR | L | 687.8 | 715.4 (y7) | 862.4 (y8) | 1025.5 (y9) | |
| H | 695.3 | 725.3 (y7) | 873.4 (y8) | 1037.5 (y9) | |||
| HDAC3 | YTGASLQGATQLNNK | L | 783.4 | 845.4 (y8) | 973.5 (y9) | 1173.6 (y11) | |
| H | 793.4 | 857.4 (y8) | 987.5 (y9) | 1189.6 (y11) | |||
| HDAC4 | ESAVASTEVK | L | 510.8 | 563.3 (y5) | 634.3 (y6) | 733.4 (y7) | |
| H | 516.2 | 569.3 (y5) | 641.3 (y6) | 741.4 (y7) | |||
| DQPVELLNPAR | L | 626.3 | 683.4 (y6) | 812.5 (y7) | 1008.6 (y9) | ||
| H | 634.3 | 693.4 (y6) | 823.4 (y7) | 1021.5 (y9) | |||
| HDAC5 | LSTQQEAER | L | 510.8 | 632.3 (y5) | 760.4 (y6) | 861.4 (y7) | |
| H | 531.3 | 641.3 (y5) | 771.3 (y6) | 873.4 (y7) | |||
| HDAC6 | EQLIQEGLLDR | L | 657.4 | 702.4 (y6) | 830.4 (y7) | 943.5 (y8) | 1056.6 (y9) |
| H | 665.3 | 711.4 (y6) | 841.4 (y7) | 955.5 (y8) | 1069.6 (y9) | ||
Transitions are listed for both unlabeled, light (L) and 15N-labeled, heavy (H) peptides. Precursor ions are +2 charge and product ions are +1 charge with y-ion included for reference.