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. 2017 Oct 26;12(10):e0187162. doi: 10.1371/journal.pone.0187162

Fig 3. Genetic activation of Nrf2 in myeloid cells.

Fig 3

(A) Schematic representation of the transgenes used for the generation of LysM-Cre CMVcaNrf2 mice. (B) Left upper panel: RNA from sorted neutrophils or macrophages from 5dw of control (tg/wt) and LysM-cre CMVcaNrf2 mice (tg/tg) were analyzed for caNrf2 transgene expression and for Rps29 by RT-qPCR. PCR products were analyzed by agarose gel electrophoresis. Other panels: RT-qPCR using RNA from lymphocytes, neutrophils and macrophages isolated from 5d wounds for the Nrf2 target genes Gclc, Srxn1, Slpi, and Nqo1 relative to Rps29. N = 3 mice. Bars indicate median with 95% CI. (C) Flow cytometry analysis of 5dw and 13dw of tg/wt and tg/tg mice for total leukocytes, myeloid cells, neutrophils and macrophages. N = 3–5 mice, n = 5–10 wounds. Bars indicate mean ±SD. *P ≤0.05; **P ≤0.01; ***P ≤0.001, **** P ≤0.0001 (Mann-Whitney U test).