Table 2. Antibodies and reagents used for flow cytometry.
| Antigen | Clone | Fluorophore | Dilution | Source |
|---|---|---|---|---|
| CD16/32 | 2.4G2 | 1:200 | BD Biosciences, Franklin Lakes, NJ | |
| CD45 | 30-F11 | BV 605 | 1:500 | BD Biosciences |
| 30-F11 | PB | 1:400 | BD Biosciences | |
| CD11b | M1/70 | PE | 1:200 | BD Biosciences |
| M1/70 | BV 711 | 1:500 | BioLegend, San Diego, CA | |
| CD3 | 17A2 | BV 785 | 1:300 | BioLegend |
| 145-2C11 | PE | 1:400 | BD Biosciences | |
| F4/80 | Cl:A3-1 | AF 647 | 1:200 | AbD Serotec, Kidlington, UK |
| Ly6C | AL-21 | PE | 1:200 | BD Biosciences |
| HK1.4 | PE-Cy7 | 1:500 | BioLegend | |
| HK1.4 | PerCP-Cy5.5 | 1:500 | BioLegend | |
| Ly6G | 1A8-Ly6g | AF 700 | 1:100 | BioLegend |
| 1A8-Ly6g | FITC | 1:200 | BD Biosciences | |
| 1A8-Ly6g | PerCP-Cy5.5 | 1:200 | BioLegend | |
| Live/Dead | Zombie Aqua | 1:400 | BioLegend |