Fig 5. hpk-1 regulates thermotolerance.

(A) hpk-1 is required for normal thermotolerance, as hpk-1(pk1393) has impaired survival at 35°C (red vs. black), which is rescued by transgenic Phpk-1::hpk-1::GFP (green). Graph represents survival data from one representative trial of three independent replica set experiments. Additional trials can be found in S2 Table; see methods and [38, 72] for additional details and statistical analysis. (B) hpk-1 overexpression increases survival to thermal stress (column 3 versus 1), dependent on hsf-1 (column 4 versus 2). Plotted data show mean and S.E.M. of a representative trial (trial 1) with 3–4 technical replicates per condition; # above each column represents total number of animals scored per condition. See S2 Table for additional details. *** indicates p-values of <0.001. P-values were calculated using ANOVA with Tukey’s HSD post-hoc, and were corrected to account for multiple testing. See S2 Table for additional trial data.