(A-D) Representative images of at least 30 Phsp-16.2::GFP animals in wild type (A, C) and hpk-1(pk1393) animals (B, D) under basal conditions (A, B) or after heat shock (C, D). Animals are outlined in panels A-B. (E-F) Induction of endogenous hsp-16.2 (E) and hsp-70 (C12C8.1) (F), in N2 and hpk-1(pk1393) animals as measured by qRT-PCR. (G) Loss of hpk-1 did not alter endogenous expression of hsp-16.2 (blue) or hsp-70 (orange). Values in (E-G) are normalized to expression of act-1 and the mean fold change relative to wild-type animals, and the S.E.M. between technical replicates is shown. In total three independent experiments were performed with similar results. P-values for (E) and (F) are <0.05 and <0.01, respectively (Student’s t-test).