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. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038

Fig 9. hpk-1 protein expression is stimulated by heat shock.

Fig 9

(A) Expression of HPK-1 protein (Phpk-1::HPK-1::GFP) under basal conditions is primarily restricted to neurons. Fluorescent intestinal speckles are non-specific gut granules[111]. (B-D) Heat shock induces HPK-1 protein (Phpk-1::HPK-1::GFP) levels most strongly within hypodermal seam cells (indicated by arrows) independent of hsf-1 (C) and transcription (D). Phpk-1::HPK-1::GFP animals after heat shock with (B) empty vector HT115, (C) hsf-1(RNAi), and (D) α-amanitin treatment. Increased HPK-1 expression within neurons and hypodermal seam cells is specific to heat stress as neither oxidative stress (E) or UV damage (F) altered expression. White space was artificially filled for some images and animals are outlined. GFP quantification and further analysis can be found in S6 Fig.