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. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038

Fig 11. HPK-1 but not HSF-1 is essential for autophagosome formation.

Fig 11

(A-F) Inactivation of hpk-1 but not hsf-1 disrupts autophagosome formation after bacterial deprivation (BD) as visualized by puncta formation for the autophagosomal reporter Plgg-1::LGG-1::GFP (Atg8p/MAP-LC3) [18]. (G) Quantification of LGG-1:GFP foci in L3 stage animals under ad libitum (AL) and bacterial deprivation (BD) conditions. BD was imposed by removal from bacterial food for 6 hours prior to scoring puncta formation. Plotted are the mean number of LGG-1::GFP puncta/seam cell visualized +/-S.D. *** indicates a p-value of <0.001 (Student’s t-test). Summary data provided in S4 Table.