Skip to main content
. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Exp Neurol. 2017 Aug 19;298(Pt A):1–12. doi: 10.1016/j.expneurol.2017.08.010

Figure 1. Sciatic nerve constriction impaired SIA, increased CRF-ir in the central amygdala and ΔFosB-ir in the dorsal horn of the spinal cord.

Figure 1

Panel A: Insertion of cuffs on the sciatic nerve decreased the mechanical paw withdrawal thresholds when compared to pre-surgical day 0 or when compared to the control group from day 5 to the end of the experiment at day 15. Two-Way ANOVA, *** - significant for interaction (time × cuff), F 3,27 = 12.4, P < 0.001 and for nerve-cuffed versus control at day 5, 10 and 15, Repeated Measures Two-Way ANOVA, F 1,9 = 41.2, P < 0.001. Panel B: Non-stressed, nerve-cuffed mice showed a normal response to thermal stimulus in a tail-flick test, but they were not able to generate SIA to the level of the control group after five minutes of forced swim. Two-Way ANOVA, *** - significant for interaction (pain × stress), F1,84 = 16.5, P < 0.001. Panels C and D: CRF-ir in the CeAmy was increased in nerve-cuffed mice (D) when compared to the controls (C); T-test, t16 = 7.2, P < 0.001. Panels E and F: Sciatic nerve constriction also increased ΔFosB-ir on the cuffed side of the dorsal horn of the spinal cord (F) when compared to the controls (E); T-test, t16 = 2.6, P < 0.05. Abbreviations: Roman numerals from I to V label the laminae of the dorsal horn. BLA -basolateral amygdala, CeC - centrocentral nucleus, CeM - cenrtromedial nucleus, CeL-centrolateral nucleus, FS – forced swim, GP - globus pallidus. Scale bar = 200 μm in D and 100 μm in F.