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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Exp Neurol. 2017 Aug 19;298(Pt A):1–12. doi: 10.1016/j.expneurol.2017.08.010

Figure 6. Injection of AAV-FLEX/DTX into the CeAmy and CAV2-cre into the LC decreased expression of CRF-ir in the CeAmy. The ablation of CRF neurons in CeAmy on the side of injury decreased the expression of ΔFosB-ir in the dorsal horn of the spinal cord in mice with sciatic nerve constriction.

Figure 6

Panel A and B: CRF-ir expression in the contralateral CeAmy (A) and ipsilateral CeAmy (B) of nerve-cuffed mice; T-test, t14 = 4.3, P < 0.001. Panel C and D: ΔFosB-ir in the dorsal horn of the spinal cord after 15 days of neuropathic pain in mice with ablation contralateral to the nerve cuff (C) and ipsilateral to the nerve cuff (D), T-test, t27 = 3.9, P < 0.001. Abbreviations: BLA - basolateral amygdala, CeC - centrocentral nucleus, CeM -cenrtromedial nucleus and CeL- centrolateral nucleus. Roman numerals from I to V indicate spinal cord laminae. Scale bar = 200 μm in A and B and scale bar = 100 μm in C and D.