Skip to main content
. 2017 Jun 26;36(43):5958–5968. doi: 10.1038/onc.2017.206

Figure 4.

Figure 4

shRNA depletion of Ror2 expression in vivo results in changes in tumor cell plasticity between basal-like and claudin-low subpopulations in the 2225L TP53-null model. (a) Representative fluorescence-activated cell sorting (FACS) plot depicting the segregation of 2225L tumors into two distinct populations based on CD24 and CD29 cell surface marker staining. (b) Quantitative real-time PCR of Ror2 and Lrp5 within sorted basal-like and claudin-low fractions from 2225L. (c) Quantitative real-time PCR of Wnt ligands within sorted basal-like and claudin-low subpopulations within 2225L. (d) FACS plots of shLUC and shRor2 tumors stained with CD24 and CD29, illustrating the shift in tumor cell populations upon Ror2 loss, from basal-like to claudin-low. (e) Quantitation of basal-like and claudin-low populations within shLUC and shRor2 2225L TP53-null tumors (shLUC basal-like 93.4±1.2% claudin-low 4.97±0.7% vs shRor2 basal-like 80.3±1.6% claudin-low 18.2±1.2%, n=5). (f) Box plots depicting gene expression signatures across subtypes represented in shRor2 vs shLUC tumors. Genes upregulated in shRor2 2225L tumors are associated with claudin-low features. Genes downregulated are associated with basal-like features (red boxes).