Fig. 5.

Anti-Ptn Ab neutralizes Ptn-induced endothelial proliferation. a Affinity-purified anti-Ptn Ab specifically recognizes Ptn in mouse retinal homogenate. ~200 μg/lane. b Proliferation assay with HRMVECs was performed as described in Fig. 1a. Ptn, 200 ng/ml; VEGF, 100 ng/ml; anti-Ptn Ab, 400 ng/ml. The assay was independently performed three times with similar outcomes. One representative experiment is shown. n = 8 wells. ±SEM, one-way ANOVA test.