Fig. 7.

Anti-Ptn Ab prevents OIR-induced pathological neovascularization. A Representative images of OIR. Anti-Ptn (0.1 μg/1 μl/eye), aflibercept (2 μg/1 μl/eye) or control IgG (0.1 μg/1 μl/eye) was intravitreally injected at P12. RNV was analyzed at P17. Arrowheads indicate neovascularization (NV) and NV tufts. Bar 500 μm. b Quantification of NV. c Quantification of NV tufts. d Quantification of branching points. e Quantification of avascular area in central retina. n = 11 eyes for anti-Ptn, 4 for aflibercept, 6 for control IgG, 4 for PBS and 8 for healthy control. ±SEM, one-way ANOVA test