Table 4.

Comparative analysis between traditional and microplate methods of siderophore estimation

Comparative analysis	Methods
	Traditional method	Microplate method
Labour	Requires high labour input	Requires less labour input
Media	96 ml	48 ml
Reagent (for 23 strains plus control in quadruplicates)	48 ml	9.6 ml
Accuracy	Less accurate because several samples are handled individually which may cause high handling error	More accurate because several samples (96) can be handled collectively in only one plate which reduces the handling error
Time	180 min (3 h)	15 min