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Mnf2 knockdown attenuates apoptosis in AML-12 hypoxia cell lines. The Annexin V/PI Apoptosis Detection Kit by flow cytometry (A and C); Caspase 3/7 detection kit and tetramethylrhodamine methyl ester perchlorate by confocal microscopy (B) used to detect apoptosis. The ratio of apoptosis cells was lower in both the NC and hypoxia + Si groups. AML: Alpha mouse liver; Mfn2: Mitofusin-2; NC: Normal culture. aP < 0.05.
