Figure 7.

Mfn2 knockdown relieves mitochondrial Ca²⁺ overloading. Rhod-2 AM was used to detect mitochondrial Ca²⁺ concentrations by confocal microscopy and VarioskanFlash. A: Confocal microscopy confirmed that the vast majority of Rhod-2 AM fluorescence was associated with the mitochondria; B: VarioskanFlash was used to detect Rhod-2 AM to determine the fluorescence of mitochondrial Ca²⁺ concentrations. Taking the hypoxia group as the reference, both the NC and hypoxia + Si groups relieved mitochondrial Ca²⁺ overloading. Mfn2: Mitofusin-2; NC: Normal culture; Rhod-2 AM: Rhod-2 acetoxymethyl ester. ^cP < 0.001.