Table 1.
Primer sequences for qRT-PCR
| Target gene | Primer sequence |
| Rat-β-Actin | Forward: 5'-ACGGTCAGGTCATCACTATCG-3' |
| Reverse: 5'-GAGGTCTTTACGGATGTCAACG-3' | |
| Rat-Mfn2 | Forward: 5'-GAAGAAGAGTGTCAAGACCGTG-3' |
| Reverse: 5'-CAGGCAAAACTTATCAATCCAG-3' | |
| Rat-Mcu | Forward: 5'-GACCCTGAACGATGTGAAGAC-3' |
| Reverse: 5'-TTCTCCGCTTTCCTGCTAAT-3' | |
| Rat-MICU-1 | Forward: 5'-GACTAAGCGGAGACTGATGTTG-3' |
| Reverse: 5'-GAGATTCTGCGTGAGCCTTC-3' | |
| Rat-MICU-2 | Forward: 5'-ACAAAGCCTCACTCTGGGT-3' |
| Reverse: 5'-TCACTGTTGGTTCCTGGTATT-3' | |
| Mus-β-Actin | Forward: 5'-CATTGCTGACAGGATGCAGAAGG-3' |
| Reverse: 5'-TGCTGGAAGGTGGACAGTGAGG-3' | |
| Mus-Mfn2 | Forward: 5'-CAAGTGTCCGCTCCTGAAG-3' |
| Reverse: 5'-CCACCAGCACAAACACATC-3' | |
| Mus-Mcu | Forward: 5'-GAGCCGCATATTGCAGTACGGT-3' |
| Reverse: 5'-AAACACGCCGACTGAGTCAGAG-3' | |
| Mus-MICU-1 | Forward: 5'-GAAGTGTCCAGCCGTGAAGGAA-3' |
| Reverse: 5'-TGGTGTGGAGTAGGCTCGGATT-3' | |
| Mus-MICU-2 | Forward: 5'-GGAAGACTTTGCTATCGCCATGC-3' |
| Reverse: 5'-GGTGTCCAGAAGATTGTCCGAG-3' |
Mfn2: Mitofusin-2; Mcu: Mitochondrial Ca2+ uniporter; MICU: Mitochondrial Ca2+ uptake.