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. 2017 Oct 26;7:14076. doi: 10.1038/s41598-017-14292-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HFD induces the increase of Vγ4⁺ γδ T cells in the skin draining lymph node and blood. (a–c) Ear swelling response in IMQ-induced psoriasis model (a), body weight (b) and the number of Vγ4⁺ γδ T cells in the whole ear skin and the draining LNs (c) of steady state ND- and HFD-fed mice at 1 and 3 week after HFD-feeding. (d) Flow cytometric analysis of the number of Vγ4⁺ γδ T cells in the whole ear skin, cervical lymph node and peripheral blood of steady state ND- and HFD-fed mice at 10 week after HFD-feeding. Results are expressed as the mean ± SD (a), SEM (b–d). p-values were obtained by Mann-Whitney-U-test. *p ≤ 0.05. Data are from one experiment, representative of independent two experiments with three to four mice (a–c), two experiments with two to three mice (d).