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. 2017 Oct 26;7:14155. doi: 10.1038/s41598-017-14469-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Length-dependent increase of specific targets and loss of genomic background shown with qPCR. In all cases the amplicon closest to the element targeted for linear enrichment showed the highest increase in signal, expressed as the relative Cq value and calculated by subtracting the Cq after LE (dark bars) or snPCR (light bars) from the Cq of the starting material. This means the relative Cq value for each starting point is zero. Panel a: Enrichment for tNOS downstream. (b) Enrichment for tNOS upstream. (c) Enrichment for p35S downstream. (d) Enrichment for p35S upstream. In all panels, the dashed bars for HMG indicate a reduction beyond detection.