Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 26;7:14167. doi: 10.1038/s41598-017-14326-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Optical Imaging of Growth over Time in Primary Culture Models (IR-783). (a) Schematic of surrogate setup utilizing primary murine mammary tumors (MMTV-neu), including dissociation of 0.5 g of tumor with a sieve, incorporation of dissociated cells into ECM, and placement into the perfusion bioreactor system. (b) Representative images from IVIS imaging of IR-783 signal in two surrogates - at days 3 and 7, or days 3 and 12 of culture - showing a stable signal over the culture period. Some variability in signal between the two surrogates at day 3 can be explained by a difference in cellularity and cell distribution in the volume of tumor incorporated into each. (c) Photomicrographs of H&E stained sections (200x magnification) from imaged surrogates containing murine primary tumors demonstrating an epithelial morphology. (d) ROI measurements of near-IR signal on days 3, 7, and 12 indicating no significant change over time (Kruskal-Wallis test, p = 0.71). (e) Measurement of histologic cell density evaluated as the number of nucleated cells per cross-sectional area showing maintenance of cell number over time (Kruskal-Wallis test, p = 0.22). n = 6 surrogates per time point (obtained from 3 murine tumors, 2 surrogates per murine tumor. Data in d & e represent mean ± SE.