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. 2017 Oct 26;7:14089. doi: 10.1038/s41598-017-14372-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Architecture of ligand-binding site in membrane-proximal module of TlpC dCACHE domain. The (mFo - DFc) σ_A-weighted electron density for lactate is shown in green. The map was calculated at 2.2 Å resolution and contoured at the 3.0 σ level. The lactate molecule is shown in all-atom ball-and-stick representation with C atoms coloured orange. The protein side chains that form direct contacts with lactate are shown in stick representation.