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. 2017 Oct 26;7:14089. doi: 10.1038/s41598-017-14372-2

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© The Author(s) 2017

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ITC titrations of TlpC LBD and its Y249F variant with lactate. The upper panel shows raw titration data, where each peak corresponds to the injection of 10 μl of 5 mM sodium L-lactate into a 1.45-ml reaction cell containing 10 μM protein. The lower panel shows the integrated and dilution-corrected peak areas of the titration plot.