Table 1.

Thermodynamic parameters of lactate binding to TlpC LBD and its variants derived from ITC measurements.

Protein	K D	Enthalpy, ∆H (cal/mol)	Entropy, ∆S (cal/mol/degree)
TlpC LBD native	155.0 ± 5.0 µM	−21,323.3 ± 713.0	−54.1 ± 2.0
TlpC LBD N213A	>3,000	—	—
TlpC LBD I218A	3.1 ± 0.6 mM	−18,145.0 ± 49.0	−49.4 ± 0.2
TlpC LBD Y249A	>3,000	—	—
TlpC LBD Y285F	>3,000	—	—
TlpC LBD F202A	>3,000	—	—
TlpC LBD K223A	>3,000	—	—
TlpC LBD S104A	359.0 ± 3.0 µM	−13,105 ± 318.0	−28.2 ± 1.0
TlpC LBD Y151A	278.5 ± 2.0 µM	−12,040 ± 250.2	−24.1 ± 1.0
TlpC LBD K153A	467.2 ± 5.0 µM	−12,730 ± 345.0	−27.5 ± 1.0