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. 2017 Oct 27;8:1153. doi: 10.1038/s41467-017-01233-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Improvement of rate limiting steps in the constructed pathway of maleate production. a The amount of maleate produced by each candidate transformant after 48 h of cultivation. Time courses of b–e the bacterial growth (filled symbols) and glucose concentration (open symbols), and f–i produced maleate (circles), 3HBA (squares), and gentisate (triangles) in the cultures of CMtS09, CMtS10, CMtS091 and CMtS101. Data are presented as the mean ± standard deviation of three independent experiments (n = 3)