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. 2017 Oct 26;7:14135. doi: 10.1038/s41598-017-14458-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Implementation and quantitative analysis of label-free single cell tracking of human primary macrophages in 3D Coll I matrices. (A) Cell migration trajectories at 17 h, 34 h, 50 h and 67 h time points. (Scale bar = 200 µm). (B) Visualization of cell migration trajectories in 3D. (C) Quantitative population analysis of mean cell migration speed distribution at all and different time points. (D) Qualitative analysis of cell migration at single cell level over 67 h cultivation time. (i) 3D visualization of single cell trajectory and (ii) single cell migration speed and morphological parameters such as roundness and aspect ratio.