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. 2017 Oct 26;7:14133. doi: 10.1038/s41598-017-14207-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunocytochemistry of glial and neuronal markers in Venus (+) cells after astrocytic differentiation. (a,c) Confocal images of the immunocytochemistry after astrocytic differentiation. Venus fluorescence is shown in green, and each glial marker is shown in red or blue. Venus (+) OPCs developed into flat shaped cells with short primary cell processes (A: arrows). Most of Venus (+) cells expressed A2B5 with GFAP or S-100, or GFAP with S-100. Arrow: punctated staining of A2B5 on Venus (+) cell; Scale bar, 25 µm. (b,d) Cell count analysis showing cell population of Venus (+) cells expressing each marker. Error bars, s.e.m.