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. 2017 Oct 26;7:14153. doi: 10.1038/s41598-017-14190-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dwell time distributions of NS SOS mutants on the cell surface. (a) Example of the change in fluorescence intensity on the cell surface, showing the association (on) and dissociation (off) of a single SOS molecule. (b) Release curves of SOS molecules from the cell surface. Molecules contained the REM(−) mutation (left) or did not (right). A total of 624–7222 molecules were observed under each condition. Black, red, and green plots show the curves obtained after stimulation with EGF for 0, 3, and 8 min, respectively. The vertical lines show the dwell times at the 10% remaining points. (c,d) Half-lives of the SOS molecules on the cell surface were plotted as indicators of the dissociation kinetics. SOS molecules contained the REM(−) mutation (d) or did not (c). Average of 7–10 cells shown with SE. Asterisks indicate a significant increase (p < 0.05 on Mann–Whitney test) compared with the WT or WT/REM(−) under the corresponding conditions.